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cell lines sf21 insect cells invitrogen  (Thermo Fisher)


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    Thermo Fisher cell lines sf21 insect cells invitrogen
    Cell Lines Sf21 Insect Cells Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sf21+insect+cell+line/pm38513655-667-237-242
    Average 86 stars, based on 1 article reviews
    cell lines sf21 insect cells invitrogen - by Bioz Stars, 2026-10
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    Cell Culture:

    Article Title: Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.
    Article Snippet: The goal of this research was to induce neuronlike properties in Sf21 cells, an insect ovarian cell line, which could lead to a new high-throughput insecticide screening method and a way to mass produce insect neuronal material for basic research.. This study applied differentiation agents to produce viable neuron-like cells.. In the presence of the molting hormone 20-hydroxyecdysone (20-HE), or insulin, in the growth medium, a maximum of ca.



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    A) A difference in activity is seen between the WT and mutant proteins using NAD + hydrolysis assay, showing that both PARP15 CAT and PARP15 FL D665A, D665R and PARP15 CAT R576A mutants are not hydrolyzing NAD + efficiently. B) PARP15 FL D665A and D665R are modified during expression in the Sf21 insect cells, showing MARylation before the incubation with NAD + in vitro , but not showing enhanced MARylation upon NAD + incubation. Full western blot available in Supplementary (Fig. S2) . C) In SEC–MALS PARP15 FL WT elutes as a dimer, whereas D665A and D665R elute as monomers. D) PARP15 FL H559Y tagged with either GFP or HA and HA-PARP15 FL H559Y D665R were expressed in HEK293 cells. GFP-tagged proteins were enriched using GFP-TRAP. Co-purified HA-tagged proteins were evaluated on western blots. For control, whole cell lysates (WCL) were analyzed for expression of the indicated proteins. E) The expression of GFP-PARP15 FL , GFP-PARP15 FL H559Y and GFP-PARP15 FL D665R was induced with doxycycline in U2OS cells and subsequently the indicated proteins were enriched using GFP-TRAPs. PARP15 expression was monitored using specific antibodies after enrichment and in WCL. MARylation was assessed in the enriched samples.

    Journal: bioRxiv

    Article Title: Dimerization of human PARP15 is required for NAD + binding and automodification

    doi: 10.64898/2025.12.15.694324

    Figure Lengend Snippet: A) A difference in activity is seen between the WT and mutant proteins using NAD + hydrolysis assay, showing that both PARP15 CAT and PARP15 FL D665A, D665R and PARP15 CAT R576A mutants are not hydrolyzing NAD + efficiently. B) PARP15 FL D665A and D665R are modified during expression in the Sf21 insect cells, showing MARylation before the incubation with NAD + in vitro , but not showing enhanced MARylation upon NAD + incubation. Full western blot available in Supplementary (Fig. S2) . C) In SEC–MALS PARP15 FL WT elutes as a dimer, whereas D665A and D665R elute as monomers. D) PARP15 FL H559Y tagged with either GFP or HA and HA-PARP15 FL H559Y D665R were expressed in HEK293 cells. GFP-tagged proteins were enriched using GFP-TRAP. Co-purified HA-tagged proteins were evaluated on western blots. For control, whole cell lysates (WCL) were analyzed for expression of the indicated proteins. E) The expression of GFP-PARP15 FL , GFP-PARP15 FL H559Y and GFP-PARP15 FL D665R was induced with doxycycline in U2OS cells and subsequently the indicated proteins were enriched using GFP-TRAPs. PARP15 expression was monitored using specific antibodies after enrichment and in WCL. MARylation was assessed in the enriched samples.

    Article Snippet: Insect cell line Sf21 was used for the expression of FL protein constructs using Bac-to-Bac® baculovirus expression system (Invitrogen).

    Techniques: Activity Assay, Mutagenesis, Hydrolysis Assay, Modification, Expressing, Incubation, In Vitro, Western Blot, Purification, Control